Structure and assembly of the NOT10:11 module of the CCR4-NOT complex

Author:

Levdansky YevgenORCID,Raisch TobiasORCID,Deme Justin C.ORCID,Pekovic FilipORCID,Elmlund Hans,Lea Susan M.ORCID,Valkov EugeneORCID

Abstract

AbstractNOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly.

Funder

U.S. Department of Health & Human Services | National Institutes of Health

Max-Planck-Gesellschaft

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

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