A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development-Reference-Cited by-同舟云学术

A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

Published:2022-03-08 Issue:1 Volume:5 Page:
ISSN:2399-3642
Container-title:Communications Biology
language:en
Short-container-title:Commun Biol

Author:

Reyes-Alcaraz Arfaxad^ORCID,Lucero Garcia-Rojas Emilio Y.,Merlinsky Elizabeth A.,Seong Jae Young,Bond Richard A.,McConnell Bradley K.^ORCID

Abstract

AbstractInternalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.

Funder

National Heart and Lung Institute

American Heart Association

Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation. We are also grateful for grant support from the University of Houston; Grants to Enhance Research on COVID-19 and the Pandemic (B.K.M).

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

Link

https://www.nature.com/articles/s42003-022-03163-9.pdf

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