Abstract
AbstractCopy number variation (CNV) is a major source of genetic variation and often contributes to phenotypic variation in maize. The duplication at the27-kDa γ-zeinlocus (qγ27) is essential to convert soft endosperm into hard endosperm in quality protein maize (QPM). This duplication is unstable and generally produces CNV at this locus. We conducted genetic experiments designed to directly measure DNA rearrangement frequencies occurring in males and females of different genetic backgrounds. The average frequency with which the duplication rearranges to single copies is 1.27 × 10−3and varies among different lines. A triplication ofγ27gene was screened and showed a better potential than the duplication for the future QPM breeding. Our results highlight a novel approach to directly determine the frequency of DNA rearrangements, in this case resulting in CNV at theqγ27locus. Furthermore, this provides a highly effective way to test suitable parents in QPM breeding.
Funder
National Natural Science Foundation of China
Chinese Ministry of Science and Technology | Department of S and T for Social Development
Publisher
Springer Science and Business Media LLC
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献