Abstract
AbstractCuttage propagation involves adventitious root formation induced by auxin. In our previous study, Larix kaempferi BABY BOOM 1 (LkBBM1), which is known to regulate adventitious root formation, was affected by auxin. However, the relationship between LkBBM1 and auxin remains unclear. Auxin response factors (ARFs) are a class of important transcription factors in the auxin signaling pathway and modulate the expression of early auxin-responsive genes by binding to auxin response elements. In the present study, we identified 14 L. kaempferi ARFs (LkARFs), and found LkARF7 and LkARF19 bound to LkBBM1 promoter and enhanced its transcription using yeast one-hybrid, ChIP-qPCR, and dual-luciferase assays. In addition, the treatment with naphthalene acetic acid promoted the expression of LkARF7 and LkARF19. We also found that overexpression of these two genes in poplar promoted adventitious root formation. Furthermore, LkARF19 interacted with the DEAD-box ATP-dependent RNA helicase 53-like protein to form a heterodimer to regulate adventitious root formation. Altogether, our results reveal an additional regulatory mechanism underlying the control of adventitious root formation by auxin.
Publisher
Springer Science and Business Media LLC
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference83 articles.
1. Pijut, P., Woeste, K. & Michler, C. Promotion of adventitious root formation of difficult‐to‐root hardwood tree species. Hortic. Rev. 38, 213–251 (2011).
2. de Klerk, G. J., van der Krieken, W. & de Jong, J. C. The formation of adventitious roots: new concepts, new possibilities. Vitr. Cell. Dev. Biol. 35, 189–199 (1999).
3. Guan, L. et al. Physiological and molecular regulation of adventitious root formation. Crit. Rev. Plant Sci. 34, 506–521 (2015).
4. Ikeuchi, M. et al. Molecular mechanisms of plant regeneration. Annu. Rev. Plant Biol. 70, 377–406 (2019).
5. Steffens, B. & Rasmussen, A. The physiology of adventitious roots. Plant Physiol. 170, 603–617 (2016).
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献