Altered transcriptional and chromatin responses to rhinovirus in bronchial epithelial cells from adults with asthma-Reference-Cited by-同舟云学术

Altered transcriptional and chromatin responses to rhinovirus in bronchial epithelial cells from adults with asthma

Published:2020-11-13 Issue:1 Volume:3 Page:
ISSN:2399-3642
Container-title:Communications Biology
language:en
Short-container-title:Commun Biol

Author:

Helling Britney A.^ORCID,Sobreira Débora R.,Hansen Grace T.^ORCID,Sakabe Noboru J.,Luo Kaixuan,Billstrand Christine,Laxman Bharathi,Nicolae Raluca I.,Nicolae Dan L.,Bochkov Yury A.,Gern James E.^ORCID,Nobrega Marcelo A.,White Steven R.,Ober Carole

Abstract

AbstractThere is a life-long relationship between rhinovirus (RV) infection and the development and clinical manifestations of asthma. In this study we demonstrate that cultured primary bronchial epithelial cells from adults with asthma (n = 9) show different transcriptional and chromatin responses to RV infection compared to those without asthma (n = 9). Both the number and magnitude of transcriptional and chromatin responses to RV were muted in cells from asthma cases compared to controls. Pathway analysis of the transcriptionally responsive genes revealed enrichments of apoptotic pathways in controls but inflammatory pathways in asthma cases. Using promoter capture Hi-C we tethered regions of RV-responsive chromatin to RV-responsive genes and showed enrichment of these regions and genes at asthma GWAS loci. Taken together, our studies indicate a delayed or prolonged inflammatory state in cells from asthma cases and highlight genes that may contribute to genetic risk for asthma.

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

Link

http://www.nature.com/articles/s42003-020-01411-4.pdf

Reference62 articles.

1. Jacobs, S. E., Lamson, D. M., St George, K. & Walsh, T. J. Human rhinoviruses. Clin. Microbiol. Rev. 26, 135–162 (2013).

2. Corne, J. M. et al. Frequency, severity, and duration of rhinovirus infections in asthmatic and non-asthmatic individuals: a longitudinal cohort study. Lancet 359, 831–834 (2002).

3. Message, S. D. et al. Rhinovirus-induced lower respiratory illness is increased in asthma and related to virus load and Th1/2 cytokine and IL-10 production. Proc. Natl Acad. Sci. USA 105, 13562–13567 (2008).

4. Traves, S. L. & Proud, D. Viral-associated exacerbations of asthma and COPD. Curr. Opin. Pharmacol. 7, 252–258 (2007).

5. Donaldson, G. C. & Wedzicha, J. A. The causes and consequences of seasonal variation in COPD exacerbations. Int. J. Chron. Obstruct Pulmon Dis. 9, 1101–1110 (2014).

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rhinovirus infection of airway epithelial cells uncovers the non-ciliated subset as a likely driver of genetic risk to childhood-onset asthma;Cell Genomics;2024-09

2. PGAP3 regulates human bronchial epithelial cell mRNAs present in asthma and respiratory virus reference data sets;2024-07-05

3. Multi-omics in nasal epithelium reveals three axes of dysregulation for asthma risk in the African Diaspora populations;Nature Communications;2024-05-28

4. Rhinovirus infection of airway epithelial cells uncovers the non-ciliated subset as a likely driver of genetic susceptibility to childhood-onset asthma;2024-02-06

5. A functional genomics pipeline to identify high-value asthma and allergy CpGs in the human methylome;Journal of Allergy and Clinical Immunology;2023-06