A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells-Reference-Cited by-同舟云学术

A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

Published:2021-06-17 Issue:1 Volume:6 Page:
ISSN:2059-0105
Container-title:npj Vaccines
language:en
Short-container-title:npj Vaccines

Author:

Funnell Simon G. P.^ORCID,Afrough Babak,Baczenas John James^ORCID,Berry Neil^ORCID,Bewley Kevin R.^ORCID,Bradford Rebecca^ORCID,Florence Clint,Duff Yann Le,Lewis Mark^ORCID,Moriarty Ryan V.^ORCID,Connor Shelby L. O.^ORCID,Osman Karen L.^ORCID,Pullan Steven,Rashid Sujatha,Richards Kevin S.^ORCID,Stemple Kimberly J.,Knezevic Ivana^ORCID

Abstract

AbstractAn array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.

Publisher

Springer Science and Business Media LLC

Subject

Pharmacology (medical),Infectious Diseases,Pharmacology,Immunology

Link

http://www.nature.com/articles/s41541-021-00346-z.pdf

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