Simulated microgravity attenuates myogenesis and contractile function of 3D engineered skeletal muscle tissues

Author:

Ren Zhanping,Ahn Eun Hyun,Do Minjae,Mair Devin B.,Monemianesfahani Amir,Lee Peter H. U.,Kim Deok-HoORCID

Abstract

AbstractWhile the effects of microgravity on inducing skeletal muscle atrophy have been extensively studied, the impacts of microgravity on myogenesis and its mechanisms remain unclear. In this study, we developed a microphysiological system of engineered muscle tissue (EMT) fabricated using a collagen / Matrigel composite hydrogel and murine skeletal myoblasts. This 3D EMT model allows non-invasive quantitative assessment of contractile function. After applying a 7-day differentiation protocol to induce myotube formation, the EMTs clearly exhibited sarcomerogenesis, myofilament formation, and synchronous twitch and tetanic contractions with electrical stimuli. Using this 3D EMT system, we investigated the effects of simulated microgravity at 10−3 G on myogenesis and contractile function utilizing a random positioning machine. EMTs cultured for 5 days in simulated microgravity exhibited significantly reduced contractile forces, myofiber size, and differential expression of muscle contractile, myogenesis regulatory, and mitochondrial biogenesis-related proteins. These results indicate simulated microgravity attenuates myogenesis, resulting in impaired muscle function.

Funder

U.S. Department of Health & Human Services | National Institutes of Health

National Science Foundation

National Aeronautics and Space Administration

Publisher

Springer Science and Business Media LLC

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