Genetically engineered synthetic cells activate cargo release upon temperature shift
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Publisher
Springer Science and Business Media LLC
Link
https://www.nature.com/articles/s41589-024-01701-6.pdf
Reference5 articles.
1. Guindani, C., da Silva, L. C., Cao, S., Ivanov, T. & Landfester, K. Synthetic cells: from simple bio-inspired modules to sophisticated integrated systems. Angew Chem. Int. Ed. Engl. 61, e202110855 (2022). This review highlights strategies used to build synthetic cells and organelles from scratch.
2. Wang, B., Barahona, M. & Buck, M. A modular cell-based biosensor using engineered genetic logic circuits to detect and integrate multiple environmental signals. Biosens. Bioelectron. 40, 368–376 (2013). This work demonstrates how a modular cell-based biosensor can be engineered using exchangeable synthetic gene circuit modules to sense and integrate multiple-input signals.
3. Garamella, J., Marshall, R., Rustad, M. & Noireaux, V. The all E. coli TX-TL toolbox 2.0: a platform for cell-free synthetic biology. ACS Synth. Biol. 5, 344–355 (2016). This paper provides a characterization of the performance and properties of an all Escherichia coli, cell-free transcription and translation system.
4. Jia, H., Heymann, M., Hartel, T., Kai, L. & Schwille, P. Temperature-sensitive protein expression in protocells. Chem. Commun. 55, 6421–6424 (2019). This paper reports the design and use of RNATs to control protein expression in liposome vesicles.
5. Sedlmayer, F., Aubel, D. & Fussenegger, M. Synthetic gene circuits for the detection, elimination and prevention of disease. Nat. Biomed. Eng. 2, 399–415 (2018). This review describes the biomedical applications of synthetic gene circuits in major disease areas, including in the clinic.
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