Multicopy suppressor screens reveal convergent evolution of single-gene lysis proteins

Author:

Adler Benjamin A.,Chamakura KarthikORCID,Carion Heloise,Krog Jonathan,Deutschbauer Adam M.ORCID,Young Ry,Mutalik Vivek K.ORCID,Arkin Adam P.ORCID

Abstract

AbstractSingle-strand RNA (ssRNA) Fiersviridae phages cause host lysis with a product of single gene (sgl for single-gene lysis; product Sgl) that induces autolysis. Many different Sgls have been discovered, but the molecular targets of only a few have been identified. In this study, we used a high-throughput genetic screen to uncover genome-wide host suppressors of diverse Sgls. In addition to validating known molecular mechanisms, we discovered that the Sgl of PP7, an ssRNA phage of Pseudomonas aeruginosa, targets MurJ, the flippase responsible for lipid II export, previously shown to be the target of the Sgl of coliphage M. These two Sgls, which are unrelated and predicted to have opposite membrane topology, thus represent a case of convergent evolution. We extended the genetic screens to other uncharacterized Sgls and uncovered a common set of multicopy suppressors, suggesting that these Sgls act by the same or similar mechanism.

Funder

Microbiology Program of the Innovative Genomics Institute, Berkeley

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology

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