Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs-Reference-Cited by-同舟云学术

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Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs

Published:2021-06-21 Issue:11 Volume:39 Page:1403-1413
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Xu Ping^ORCID,Liu Zhiheng^ORCID,Liu Ying,Ma Huazheng,Xu Yiyuan,Bao Ying,Zhu Shiyou,Cao Zhongzheng,Wu Zeguang,Zhou Zhuo,Wei Wensheng^ORCID

Funder

National Science Foundation of China | Key Programme

Beijing Municipal Science and Technology Commission

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

https://www.nature.com/articles/s41587-021-00944-1.pdf

Reference71 articles.

1. Chang, N. et al. Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos. Cell Research 23, 465–472 (2013).

2. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013).

3. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl Acad. Sci. USA 109, E2579–E2586 (2012).

4. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).

5. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013).

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