A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae

Author:

Simoni AlekosORCID,Hammond Andrew M.ORCID,Beaghton Andrea K.,Galizi Roberto,Taxiarchi Chrysanthi,Kyrou KyrosORCID,Meacci Dario,Gribble Matthew,Morselli Giulia,Burt Austin,Nolan Tony,Crisanti AndreaORCID

Abstract

AbstractOnly female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10–14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.

Funder

Bill and Melinda Gates Foundation

EC | Horizon 2020 Framework Programme

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

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