Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing-Reference-Cited by-同舟云学术

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Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

Published:2020-03-30 Issue:8 Volume:38 Page:954-961
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Replogle Joseph M.^ORCID,Norman Thomas M.,Xu Albert^ORCID,Hussmann Jeffrey A.^ORCID,Chen Jin,Cogan J. Zachery,Meer Elliott J.,Terry Jessica M.,Riordan Daniel P.,Srinivas Niranjan,Fiddes Ian T.,Arthur Joseph G.,Alvarado Luigi J.^ORCID,Pfeiffer Katherine A.,Mikkelsen Tarjei S.^ORCID,Weissman Jonathan S.^ORCID,Adamson Britt^ORCID

Funder

U.S. Department of Health & Human Services | National Institutes of Health

Damon Runyon Cancer Research Foundation

Howard Hughes Medical Institute

Jane Coffin Childs Memorial Fund for Medical Research

10x Genomics

United States Department of Defense | Defense Advanced Research Projects Agency

Chan Zuckerberg Initiative

Princeton University

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://www.nature.com/articles/s41587-020-0470-y.pdf

Reference37 articles.

1. Packer, J. & Trapnell, C. Single-cell multi-omics: an engine for new quantitative models of gene regulation. Trends Genet. 34, 653–665 (2018).

2. Feldman, D. et al. Optical pooled screens in human cells. Cell. 179, 787–799.e17 (2019).

3. Rubin, A. J. et al. Coupled single-cell CRISPR screening and epigenomic profiling reveals causal gene regulatory networks. Cell. 176, 361–376.e17 (2018).

4. Adamson, B. et al. A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response. Cell. 167, 1867–1882.e21 (2016).

5. Dixit, A. et al. Perturb-Seq: dissecting molecular circuits with scalable single-cell RNA profiling of pooled genetic screens. Cell. 167, 1853–1866.e17 (2016).

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