Profiling the total single-cell transciptome using droplet microfluidics
Author:
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
https://www.nature.com/articles/s41587-022-01370-7.pdf
Reference5 articles.
1. Aldridge, S. & Teichmann, S. A. Single cell transcriptomics comes of age. Nat. Commun. 11, 4307 (2020). This Review article describes the state of the art and challenges in transcriptomics.
2. Hagemann-Jensen, M. et al. Single-cell RNA counting at allele and isoform resolution using Smart-seq3. Nat. Biotechnol. 38, 708–714 (2020). This paper reports Smart-seq3, the method previously claimed to have the highest information content per profiled cell.
3. Zheng, G. X. Y. et al. Massively parallel digital transcriptional profiling of single cells. Nat. Commun. 8, 14049 (2017). This paper reports the widely used commercial 10x Genomics Chromium droplet platform.
4. Abate, A. et al. High-throughput injection with microfluidics using picoinjectors. Proc. Natl Acad. Sci. USA 107, 45 (2010). A microfluidic method enables the injection of an aqueous phase in the droplets to enable multistep reactions at high throughput.
5. Pijuan-Sala, B. et al. A single-cell molecular map of mouse gastrulation and early organogenesis. Nature 566, 490–495 (2019). A reference dataset generated with 10x Genomics Chromium is used for atlas benchmarking.
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