Identifying and mitigating bias in next-generation sequencing methods for chromatin biology
Author:
Publisher
Springer Science and Business Media LLC
Subject
Genetics (clinical),Genetics,Molecular Biology
Link
http://www.nature.com/articles/nrg3788.pdf
Reference119 articles.
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2. Johnson, D., Mortazavi, A., Myers, R. & Wold, B. Genome-wide mapping of in vivo protein–DNA interactions. Science 80, 1497–1502 (2007).
3. Mikkelsen, T. S. et al. Genome-wide maps of chromatin state in pluripotent and lineage-committed cells. Nature 448, 553–560 (2007).
4. Kharchenko, P. V., Tolstorukov, M. Y. & Park, P. J. Design and analysis of ChIP–seq experiments for DNA-binding proteins. Nature Biotech. 26, 1351–1359 (2008). This study proposes using the distribution of oriented reads to discriminate between real TF binding sites and artefacts.
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