CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology
Link
http://www.nature.com/articles/s41422-018-0022-x.pdf
Reference13 articles.
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2. Li, S. Y., Zhao, G. P. & Wang, J. C-brick: a new standard for assembly of biological parts using Cpf1. ACS Synth. Biol. 5, 1383–1388 (2016).
3. Lei, C. et al. The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro. Nucleic Acids Res. 45, e74 (2017).
4. Yang, H., Gao, P., Rajashankar, K. R. & Patel, D. J. PAM-dependent target DNA recognition and cleavage by C2c1 CRISPR-Cas endonuclease. Cell 167, 1814–1828 (2016).
5. Swarts, D. C., van der Oost, J. & Jinek, M. Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a. Mol. Cell 66, 221–233 (2017).
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