Infection with novel coronavirus (SARS-CoV-2) causes pneumonia in Rhesus macaques

Author:

Shan ChaoORCID,Yao Yan-Feng,Yang Xing-Lou,Zhou Yi-Wu,Gao Ge,Peng Yun,Yang Lian,Hu Xue,Xiong Jin,Jiang Ren-Di,Zhang Hua-Jun,Gao Xiao-Xiao,Peng Cheng,Min Juan,Chen Ying,Si Hao-Rui,Wu Jia,Zhou Peng,Wang Yan-Yi,Wei Hong-Ping,Pang Wei,Hu Zheng-Fei,Lv Long-Bao,Zheng Yong-TangORCID,Shi Zheng-LiORCID,Yuan Zhi-MingORCID

Abstract

AbstractThe 2019 novel coronavirus (SARS-CoV-2) outbreak is a major challenge for public health. SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases, with a mortality rate of ~6.4% worldwide (based on World Health Organization daily situation report). However, the dynamics of viral infection, replication and shedding are poorly understood. Here, we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2. After intratracheal inoculation, the first peak of viral RNA was observed in oropharyngeal swabs one day post infection (1 d.p.i.), mainly from the input of the inoculation, while the second peak occurred at 5 d.p.i., which reflected on-site replication in the respiratory tract. Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals, characterized by hyperemia and edema, and infiltration of monocytes and lymphocytes in alveoli. We also identified SARS-CoV-2 RNA in respiratory tract tissues, including trachea, bronchus and lung; and viruses were also re-isolated from oropharyngeal swabs, bronchus and lung, respectively. Furthermore, we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2. The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics.

Funder

National Key R&D Program

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology

Reference19 articles.

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