Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
Author:
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
http://www.nature.com/articles/nbt.3528.pdf
Reference47 articles.
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3. Chothia, C., Gough, J., Vogel, C. & Teichmann, S.A. Evolution of the protein repertoire. Science 300, 1701–1703 (2003).
4. Jinek, M. et al. A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).
5. Doudna, J.A. & Charpentier, E. The new frontier of genome engineering with CRISPR-Cas9. Science 346, 1258096 (2014).
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