A novel mechanism for direct real-time polymerase chain reaction that does not require DNA isolation from prokaryotic cells
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep28000.pdf
Reference31 articles.
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2. Pinhata, J. M. et al. Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory. J. Med. Microbiol. 64, 1040–1045 (2015).
3. Wood, R. C. et al. Detection of Mycobacterium tuberculosis DNA on the oral mucosa of tuberculosis patients. Sci. Rep. 5, 8668 (2015).
4. O’Mahony, J. & Hill, C. Rapid real-time PCR for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk. Appl. Environ. Microbiol. 70, 4561–4568 (2004).
5. Tasara, T. & Stephan, R. Development of an F57 sequence-based real-time PCR assay for detection of Mycobaterium avium subsp. paratuberculosis in milk. Appl. Environ. Microbiol. 71, 5957–5968 (2005).
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