An improved pathway for autonomous bioluminescence imaging in eukaryotes-Reference-Cited by-同舟云学术

An improved pathway for autonomous bioluminescence imaging in eukaryotes

Published:2024-01-22 Issue: Volume: Page:
ISSN:1548-7091
Container-title:Nature Methods
language:en
Short-container-title:Nat Methods

Author:

Shakhova Ekaterina S.^ORCID,Karataeva Tatiana A.,Markina Nadezhda M.,Mitiouchkina Tatiana,Palkina Kseniia A.,Perfilov Maxim M.^ORCID,Wood Monika G.^ORCID,Hoang Trish T.,Hall Mary P.,Fakhranurova Liliia I.,Alekberova Anna E.,Malyshevskaia Alena K.,Gorbachev Dmitry A.,Bugaeva Evgenia N.,Pletneva Ludmila K.,Babenko Vladislav V.,Boldyreva Daria I.,Gorokhovatsky Andrey Y.,Balakireva Anastasia V.,Gao Feng,Choob Vladimir V.,Encell Lance P.,Wood Keith V.,Yampolsky Ilia V.^ORCID,Sarkisyan Karen S.^ORCID,Mishin Alexander S.^ORCID

Abstract

AbstractThe discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.

Funder

RCUK | Medical Research Council

Planta

Promega

Light Bio

Russian Science Foundation

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

Link

https://www.nature.com/articles/s41592-023-02152-y.pdf

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