Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging
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Published:2023-07-19
Issue:8
Volume:20
Page:1174-1178
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ISSN:1548-7091
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Container-title:Nature Methods
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language:en
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Short-container-title:Nat Methods
Author:
Quardokus Ellen M.ORCID, Saunders Diane C.ORCID, McDonough ElizabethORCID, Hickey John W.ORCID, Werlein ChristopherORCID, Surrette Christine, Rajbhandari Presha, Casals Anna Martinez, Tian HuaORCID, Lowery Lisa, Neumann Elizabeth K., Björklund Frida, Neelakantan Taruna V., Croteau JoshORCID, Wiblin Anne E.ORCID, Fisher JeremyORCID, Livengood April J., Dowell Karen G., Silverstein Jonathan C.ORCID, Spraggins Jeffrey M.ORCID, Pryhuber Gloria S.ORCID, Deutsch Gail, Ginty Fiona, Nolan Garry P., Melov SimonORCID, Jonigk DannyORCID, Caldwell Michael A., Vlachos Ioannis S.ORCID, Muller WernerORCID, Gehlenborg NilsORCID, Stockwell Brent R.ORCID, Lundberg EmmaORCID, Snyder Michael P.ORCID, Germain Ronald N.ORCID, Camarillo Jeannie M., Kelleher Neil L.ORCID, Börner KatyORCID, Radtke Andrea J.ORCID
Abstract
AbstractMultiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Biochemistry,Biotechnology
Reference26 articles.
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