High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

Author:

Bevilacqua CarloORCID,Gomez Juan Manuel,Fiuza Ulla-MajORCID,Chan Chii Jou,Wang LingORCID,Hambura SebastianORCID,Eguren Manuel,Ellenberg JanORCID,Diz-Muñoz AlbaORCID,Leptin Maria,Prevedel RobertORCID

Abstract

AbstractBrillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

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