Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus-Reference-Cited by-同舟云学术

Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus

Published:2021 Issue:1 Volume:37 Page:26-36
ISSN:2500-2341
Container-title:Biotekhnologiya
language:ru
Short-container-title:Biiotehnologiâ (Mosk.)

Author:

Fokina V.V.¹,Tarlachkov S.V.²,Machulin A.V.³,Shutov A.A.¹,Donova M.V.¹

Affiliation:

1. 1Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russia 2Pharmins, Ltd, Pushchino, Moscow Oblast, 142290, Russia

2. 1Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russia; 2Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russia

3. Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences Federal Research Center, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290, Russia

Abstract

The identification of the Streptomyces sp. VKM Ac-2618D strain had been carried out and its morphological and physiological features have been studied in relation to the immunosuppressant tacrolimus production. The phenotypic variability of the strain was analyzed and the dissociant with a high level of tacrolimus production was identified. Based on a comprehensive study of morphological, physiological and chemotaxonomic properties, as well as phylogenetic analysis, the strain was named Streptomyces tsukubensis VKM Ac-2618D. The strain genome contains the full version of the tacrolimus biosynthetic gene cluster. The advantages of fed-batch cultivation mode for tacrolimus biosynthesis were shown. The results obtained expand the understanding of the peculiarities of polyketide biosynthesis and can be used in the development of a tacrolimus production technology. Key words: Streptomyces tsukubensis, tacrolimus, FK-506, dissociant, genome, biosynthesis The research was carried out within the framework of the state assignment АААА-А16-116062110077-6.

Publisher

National Research Center Kurchatov Institute

Subject

Ecology,Applied Microbiology and Biotechnology,Biotechnology

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