Affiliation:
1. State Research Institute of Highly Pure Biopreparations, St.-Petersburg, 197110 Russia
Abstract
Conditions for analyzing sialylated isoforms of recombinant follicle-stimulating hormone (FSH) by the method of capillary zone electrophoresis have been selected. Follicle-stimulating hormone is a glycosylated heterodimer with the large number of sialic acids that affect its basic biological and physicochemical properties. The optimum protein separation into 6 isoforms was achieved using as a background electrolyte of a solution with pH 4.3 containing 0.01 M trycin, 0.01 sodium chloride, 7 M urea and 2.5 mM 1,4-diaminobutane.
follicle-stimulating hormone isoforms, capillary zone electrophoresis, sialic acids, follicle-stimulating hormone.
Publisher
National Research Center Kurchatov Institute
Subject
Ecology,Applied Microbiology and Biotechnology,Biotechnology
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