Affiliation:
1. State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center «Kurchatov Institute» (NRC «Kurchatov Institute»-GosNIIgenetika), Moscow, 117545 Russia
Abstract
In recent years, mycelial fungi have faced competition from recombinant yeast producers in the production of feed enzymes. An intensive study on genetic diversity identified yeast genes encoding feed enzymes the specific activity of which is much higher than that in mycelial fungi. In addition, these genes were expressed in yeast much more efficiently than in mycelial fungi. The use of yeast recombinant producers allowed expanding the production of a line of industrial enzymes with a significant reduction in their cost. The advantages of yeast producers of recombinant enzymes include the possibility of obtain monoenzymes that are part of various enzyme complexes used for different purposes. Pichia pastoris methylotrophic yeast is the most attractive subject for the creation of recombinant protein-producing strains.
feed enzymes, Pichia pastoris, phytase, xylanase, β-glucanase, AOX1 promoter
The work was carried out using the equipment of the Multipurpose Scientific Installation of «All-Russian Collection of Industrial Microorganisms» National Bio-Resource Center, NRC «Kurchatov Institute» - GOSNIIGENETIKA
The work was funded by the Ministry of Education and Science of Russia (Project Unique Identifier RFMEFI60717X0180).
Publisher
National Research Center Kurchatov Institute
Subject
Ecology,Applied Microbiology and Biotechnology,Biotechnology
Reference52 articles.
1. 1. Choct M. Enzymes for the feed industry: past, present and future. World Poult. Sci. J., 2006, 62(1), 5-16.
2. 2. Egli I., Davidsson L., Juillerat M.A., et al. The influence of soaking and germination on the phytase activity and phytic acid content of grains and seeds potentially useful for complementary feeding. J. Food Sci., 2002, 67(9), 3484-3488.
3. 3. Goswami G.K., Rawat S. Microbial xylanase and their applications: a review. Int. J. Curr. Res. Aca. Rev., 2015, 3(6), 436-450.
4. 4. Butt M.S., Tahir-Nadeem M., Ahmad Z., et al. Xylanases in baking industry. Food Technol. Biotechnol., 2008, 46(1) 22-31.
5. 5. Odeniyi O.A., Onilude A.A., Ayodele M.A., Characteristics of a β-1,4-D-endoglucanase from Trichoderma virens wholly applied in a palm-fruit husk-based diet for poultry layers. Braz J Microbiol., 2012, 43(4):1467-1475.