Participation of Laminin α5-Chain in the Regulation of Colorectal Cancer Cell Differentiation-Reference-Cited by-同舟云学术

Participation of Laminin α5-Chain in the Regulation of Colorectal Cancer Cell Differentiation

Published:2019 Issue:6 Volume:35 Page:3-11
ISSN:2500-2341
Container-title:Biotekhnologiya
language:ru
Short-container-title:Biiotehnologiâ (Mosk.)

Author:

Raigorodskaya M.P. Raigorodskaya¹,Tsypina I.M.²,Turchinovich A.³,Zgoda V.G.⁴,Nikulin S.V.⁵

Affiliation:

1. Bioclinicum Scientific Research Center, Moscow, 115088 Russia

2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia,Higher School of Economics National Research University, Moscow, 101000 Russia

3. Molecular Epidemiology C080, German Cancer Research Center, Heidelberg, 69120 Germany,SciBerg e.Kfm, Mannheim, 68309 Germany

4. Institute of Biomedical Chemistry, Moscow, 119121 Russia

5. Bioclinicum Scientific Research Center, Moscow, 115088 Russia, Far Eastern Federal University, Vladivostok, 690091 Russia

Abstract

The effect of endogenous expression of the laminin α5 chain on the phenotype of colorectal cancer cells has been studied. For this purpose, the knockdown of LAMA5 gene coding for the α5 laminin chain in the HT29 colorectal adenocarcinoma cell line was carried out. The data on gene expression at both mRNA and protein levels indicate that the α5 laminin chain is involved in the regulation of cell differentiation. For example, a decrease in the expression of such markers of differentiation of the intestinal epithelium as SI, KRT20, ANXA13 and MUC13 was observed. In addition, the expression of the intestinal epithelial stem cell marker LRG5 and CD44 transmembrane protein was increased. Changing the phenotype of HT29 cells may be a result of the effect of the laminin α5 chain on the activity of the Wnt signaling pathway. colorectal cancer, laminin, differentiation, dedifferentiation, intestinal epithelium stem cell marker, HT29, LAMA5, LRG5 The study was funded by the Russian Science Foundation (Project no. 17-14-01338).

Publisher

National Research Center Kurchatov Institute

Subject

Ecology,Applied Microbiology and Biotechnology,Biotechnology

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