Affiliation:
1. Volgograd State University of the Ministry of Education and Science of the Russian Federation, Volgograd, 400062 Russia
Abstract
Abstract-The algorithm of the virtual database screening for the detection of proteins with the practical significance for the pharmaceutical and biotechnological industries has been developed. The Pythom programming language v. 3.6.5 in Notepad++ framework was used to develop the algorithm. The UniProt database served as a source of the information about the structure of the proteins comprising the bovine and pig lung proteome, and the open DrugBank database was used to the subsequent search for matches in the protein structures. The virtual screening allowed to detect more than 5,500 proteins which are present in the proteome of bovine and pig lungs; the assessment of the practical significance was absent in 99% of the proteins, although it resulted from the manual search in the DrugBank database that some of them were parts of drags. The algorithm also made it possible to find out target proteins for drags in the human lung proteome, which were similar with those contained in the bovine (46) and pig (84) lung proteome. Paired alignment of amino acid sequences was used to compare the human and animals' target proteins. In the end, the developed algorithm for virtual screening allowed to identify in the first approximation the proteins with practical significance that are in varying degrees included in the farm animals' lung proteome. In the future, the more detailed screening will be possible due to the algorithm optimization and use of closed databases, which will provide more complete information about practically valuable proteins for biotechnology and medicine.
proteome, database, DragBank, UniProt, virtual screening, Python, lungs
The work was carried out with financial support by Russian Foundation for Fundamental Research and the administration of the Volgograd region within the framework of the scientific project No. 18-44-343003
Publisher
National Research Center Kurchatov Institute
Subject
Ecology,Applied Microbiology and Biotechnology,Biotechnology
Reference20 articles.
1. Musostov Sh.A. Management of forming a strategy of development of an agro-industrial enterprise. Regional problems of economic transformation, 2015. 11(61), 96-104
2. 2. Faustino M., Veiga M., Sousa P., et al. Agro-Food Byproducts as a New Source of Natural Food Additives. Molecules, 2019, 24(6), 1056. doi: 10.3390/molecules24061056
3. 3. Sarker M., Jackman D., Booth V. Lung surfactant protein A (SPA) interactions with model lung surfactant lipids and an SP-B fragment. Biochemistry, 2011. 50(22), 4867-4876. doi: 10.1021/bi200167d
4. 4. Casals C, Cañadas O. Role of lipid ordered/disordered phase coexistence in pulmonary surfactant function. Biochim. Biophys. Acta, 2012, 1818(11), 2550-2562. doi: 10.1016/j.bbamem.2012.05.024
5. 5. Agrawal V., Smart K., Jilling T., Hirsch E. Surfactant protein (SP)-A suppresses preterm delivery and inflammation via TLR2, PLoS One, 2013, 8(5), e63990 doi: 10.1371/journal.pone.0063990