Comparative Analysis of the Binding Efficiency of Tumor-Targeting Phage Particles to Cancer Cells and Health Brain Cells

Abstract

The binding efficiency of tumor-targeting phage particles, which exhibited tumor-addressing peptides and were obtained by phage display, to the human glioblastma cell line U-87 MG and health brain cells DKM- 5 has been evaluated by flow cytometry and enzyme-linked immunosorbent assay (ELISA). The specific binding of the selected bacteriophages to U-87 MG cells was shown by fluorescence microscopy. Based on the obtained data, tumor-targeting phage particles were selected that provide the most efficient binding to the U-87 MG cells in vitro for further studies in order to create targeted antitumor compounds. phage display, tumor-addressing peptides, glioblastoma, flow cytometry, ELISA The authors are grateful to S. I. Baiborodin, Head of the Microscopic Analysis of Biological Objects Core Facilities Center, SB RAS, for assistance in conducting confocal microscopy. This study was supported by the Russian Science Fund (grant no. 19-44-02006).