Effects of fibroblast growth factor-2 and other microsupplements on the productivity of IgG and IgA producing cell lines

Abstract

Recently, serum-free media have been developed and successfully used for the cultivation of various eukaryotic cell lines. At present, along with antibodies of the IgGl isotype, therapeutic recombinant IgA antibodies are appearing in increasing frequency. However, the procedure of cultivation of cell lines producing this class of antibodies has not been optimized yet. For this reason, effects of several compounds used as additives in the culture medium on a growth and productivity of cell lines expressing antibodies were analyzed. A supplement containing the zinc salt and fibroblast growth factor-2 was shown to have stimulating effect on immunoglobulin producing cells during their cultivation in basic media, DMEM and IMDM. A complex additive, comprising dextran sulfate and iron citrate, increased the productivity of stable cell lines producing IgG and IgA antibodies and improved the homogeneity and density of cell cultures, which is especially important when producing antibodies of the IgA class. serum-free medium, microsupplements, recombinant antibodies, fibroblast growth factor-2 This work was supported by the Ministry of Science and Higher Education of Russian Federation (grant No 14.607.21.0177, ID: RFMEFI60717X0177).