Affiliation:
1. A. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar, Voronezh, 396030 Russia
Abstract
The flowering time control gene of various sugar beet plants has been studied. The BTC1 gene is a regulator for the suppressor (flowering time 1) and inducer (flowering time 2) genes of this physiological process. The F9/R9 primer pair was used for polymerase chain reaction; these primers are specific to the BTC1 gene region containing exon 9, as well as intron and exon 10. For the first time, nucleotide substitutions in exon 10 of BTC1 gene were identified in bolting sensitive samples (HF1 and BF1), which led to a change in the amino acid composition of the coded polypeptide chain. Based on the results of bioinformatic analysis, it can be assumed that certain nucleotide polymorphisms in the BTC1 gene may determine with a high probability the predisposition of sugar beet genotypes to early flowering. The use of the Geneious Prime tool for the analysis of the BTC1 gene sequences may allow the culling of genotypes prone to early flowering at early stages of selection.
sugar beet, flowering gene, BTC1, genetic polymorphism, PCR, molecular genetic markers, selection
Publisher
National Research Center Kurchatov Institute
Subject
Ecology,Applied Microbiology and Biotechnology,Biotechnology
Reference16 articles.
1. Munerati О., Reihe A. L'eredita della tendenza alla annualita nella commune barbabietola coltivata. Zeitschrift für Züchtung, Pflanzenzüchtung, 1931, 17, 84-89.
2. 2. Abegg F.A. A genetic factor for the annual habit in beets and linkage relationship. J. Agr. Res., 1936, 53, 493-511.
3. 3. Pin P., Zhang W., Vogt S., et al. The role of a pseudo-response regulator gene in life cycle adaptation and domestication of beet. Curr. Biol., 2012, 22, 1095-1101. doi: 10.1016/j.cub.2012.04.007
4. 4. Biscarini F., Stevanato P., Broccanello C., et al. Genomic predictions for binomial traits in sugar beet populations. BMC Genet., 2014, 87(15), 3-9. doi: 10.1186/1471-2156-15-87
5. 5. Kagami H., Kurata M., Matsuhira H., et al. Sugar beet (Beta vulgaris L.). Methods Mol. Biol., 2015, 1223, 335-347. doi: 10.1007/978-1-4939-1695-5_27
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