Antitumor Activity of Indole-3-carbinol in Breast Cancer Cells: Phenotype, Genetic Pattern, DNA Methylation Inversion-Reference-Cited by-同舟云学术

Antitumor Activity of Indole-3-carbinol in Breast Cancer Cells: Phenotype, Genetic Pattern, DNA Methylation Inversion

Published:2020 Issue:2 Volume:36 Page:43-55
ISSN:2500-2341
Container-title:Biotekhnologiya
language:ru
Short-container-title:Biiotehnologiâ (Mosk.)

Author:

Muyzhnek E.L.¹,Nikulin S.V.²,Kaprin A.D.³,Ashrafyan L.A.⁴,Rozhkova N.I.³,Labazanova P.G.³,Kiselev V.I.⁴

Affiliation:

1. Russian Scientific Center of Roentgenology and Radiology, Moscow, 117997 Russia

2. Bioclinicum Scientific Research Center, Moscow, 115088 Russia

3. P.A. Hertzen Moscow Oncology Research Center, National Medical Research Radiological Center, Moscow, 125284 Russia

4. National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Moscow, 117997 Russia

Abstract

The effect of indole-3-carbinol on a number of functions and characteristics of MDA-MB-231 breast cancer cells and MCF-10A healthy breast tissue cells has been studied. It was shown that indole-3-carbinol significantly reduced the proliferation and migration of MDA-MB-231 cells and does not affect these functions in MCF-10A cells. Incubation of MDA-MB-231 tumor cells with indole-3-carbinol (100 uM) for 48 h resulted in a marked decrease in the expression of the Wnt cascade genes, CCND1 (by 28%), SP1 (by 44%), CDK6 (by 47%), as well as EGFR (by 64%) and FASN (by 22%) genes. Incubation of MCF-10A cell line under the same conditions induced a noticeable decrease in expression of only two genes, EGFR (by 16%) and CDK6 (by 9%). In addition, indole-3-carbinol was also shown to manifest a selective DNA demethylation activity in breast tumor cells and to reverse the process of abnormal methylation and functional blockage of the anti-tumor WIF1 gene. These data indicate that drags containing indole-3-carbinol as an active component can be potential regulators of epigenetic processes in the treatment of breast cancer and other tumors. indole-3-carbinol, breast cancer, epigenetics, Wnt

Publisher

National Research Center Kurchatov Institute

Subject

Ecology,Applied Microbiology and Biotechnology,Biotechnology

Reference71 articles.

1. 1. Kaprin A.D., Starinskii V.V., Petrova G.V. The status of cancer care for the population of Russia in 2017. Moscow: P. Hertsen Moscow Oncology Research Institute, 2018. 235 p. (in Russian)

2. 2. Dobrokachestvennye zabolevaniya molochnoi zhelezy. Etio-patogenez, diagnostika, lechenie, profilaktika [Benign breast disease. Etiopathogenesis, diagnosis, treatment, prevention]. Eds A.D. Kaprin, N.I. Rozhkova. Moscow: «GEOTAR-Media» Publ., 2017, 283 p. (in Russian).

3. 3. Rasskazova E.A., Rozhkova N.I. Screening for early detection of breast cancer. Research and Practical Medicine Journal, 2014, 1(1), 45-51 (in Russian).

4. 4. Rozhkova N.I. Luchevaya diagnostika v mammologii [Radiology in mammology]. Moscow: «SIMK» Publ., 2014, 128 p. (in Russian).

5. 5. Profilaktika - prioritet klinicheskoi mammologii [Prevention is the priority of clinical mammology]. Eds N. I. Rozhkova, A. D. Kaprin. Moscow: «SIMK» Publ.; 2015, 188 p. (In Russian).