Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii-Reference-Cited by-同舟云学术

Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

Published:2020 Issue:6 Volume:36 Page:78-86
ISSN:2500-2341
Container-title:Biotekhnologiya
language:ru
Short-container-title:Biiotehnologiâ (Mosk.)

Author:

Akentyev P.I.¹,Gubaidullin I.I.²,Zhiganov N.I.³,Tereshchenkova V.F.⁴,Elpidina E.N.⁵,Kozlov D.G.⁶

Affiliation:

1. National Research Center «Kurchatov Institute» - GOSNIIGENETIKA, Kurchatov Genomic Center, Moscow, 117545, Russia

2. National Research Center «Kurchatov Institute», Moscow, 123182, Russia;National Research Center «Kurchatov Institute» - GOSNIIGENETIKA, Kurchatov Genomic Center, Moscow, 117545, Russia

3. Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia

4. Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia

5. A.N. Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia

6. National Research Center «Kurchatov Institute», Moscow, 123182, Russia

Abstract

A strain of the Komagataella kurtzmanii yeast, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of the pro-enzyme secretion was 20-50 mg/L. It was shown that, during secretion in yeast, the target His6-tagged protein was produced in two forms. One of them was a monomer that was efficiently purified by Ni-NTA chromatography and then activated with trypsin. Another form accumulated in the culture medium as oligomers prone to aggregation in the presence of Ni2+ ions and was not activated by trypsin treatment. Aggregation is likely the result of incorrect folding of the polypeptide chain. Tenebrio molitor, S1 family serine peptidase, SerP38, yeast, Komagataella kurtzmanii, ion-dependent aggregation

Publisher

National Research Center Kurchatov Institute

Subject

Ecology,Applied Microbiology and Biotechnology,Biotechnology

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