Affiliation:
1. Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka 812–8582, Japan
Abstract
Abstract
Background
Ischaemic preconditioning, i.e. preparatory brief ischaemia before subsequent long ischaemia, can effectively protect the heart from ischaemia–reperfusion injury in animals. The purpose of this study was to demonstrate the same phenomenon in the liver.
Methods
Using warm ischaemia–reperfusion of 70 per cent of the liver followed by resection of the non-ischaemic portion in rats, livers with 10 min of ischaemic preconditioning, i.e. 10 min of warm ischaemia and reperfusion, were compared with those that had not been subjected to such a manoeuvre.
Results
At 120 min after reperfusion following 40 min of warm ischaemia, the livers with 10 min of ischaemic preconditioning had a significantly lower mean (s.d.) serum alanine aminotransferase level (492 (217) versus 1236 (695) units/l; P < 0·005) and lactic dehydrogenase level (7905 (4002) versus 15 066 (9201) units/l; P < 0·05), as well as a higher bile output (0·12 (0·03) versus 0·09 (0·04) ml per g liver; P < 0·05) and liver tissue adenosine 5§-triphosphate level (78 (13) versus 61 (11) per cent; P < 0·05) than the control livers. The necrosis rate, histologically defined as the percentage of necrotic area in given liver sections, was reduced significantly by this manoeuvre (mean (s.d.) 1·3 (1·3) versus 5·3 (1·7) per cent; P < 0·05).
Conclusion
Ischaemic preconditioning exerts a protective effect on hepatic warm ischaemia–reperfusion injury. Such a manoeuvre may be useful for hepatic resection in the clinical setting.
Publisher
Oxford University Press (OUP)
Cited by
81 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献