Evaluation of Effect of β-Glucan on Cancer Cell Lines <i>In vitro</i>

Author:

Al-Khuzaay Hiba MuhammedORCID,Al-Juraisy Yasir Hussein,Alwan Ali Hussein,Tousson Ehab

Abstract

β-Glucan is linear polysaccharides containing d-glucose monomers connected by β-glycosidic linkages. Their structural variances are the result of several sources. This research project was designed to assess the anticancer activity by using β-glucan. The in vitro experiment employed breast cancer cell lines from Michigan Cancer Foundation-7 (MCF-7) and Ahmed, Murtudha, Jabriyah, 2013 (AMJ13). After 24, 48, and 72 hours in micro titration plate under completely sterile condition. Different concentrations of β-glucan (31.25, 62.5, 125, 250, 500, and 1000 μg/mL) were applied to the cancer cell lines. The MTT assay was used to check whether the cells had been inhibited. Cell viability in MCF7 and AMJ13 cells was significantly reduced by β-glucan. β-Glucan showed concentration and time-dependent growth inhibitory effects. The higher concentrations of β-glucan significantly (P<0.05) decrease the growth rate of cells, indicating that the higher concentrations were more effective at inhibiting growth.

Publisher

Al-Mustansiriyah Journal of Science

Reference24 articles.

1. J. Ferlay, M. Ervik, F. Lam, M. Colombet, L. Mery & M. Piñeros, Global Cancer Observatory: Cancer Today. Lyon: International Agency for Research on Cancer, 2022.

2. H. M. Al-Khuzaay, Y. H. Aljuraisy & M. M. Al-Halbosiy, The activity of aqueous extracts of leaves and roots of dandelion on cancer cell lines. Plant archive, 2019, vol. 19, no. 2, pp. 3933-3936

3. WHO-fact sheet. 2021.

4. N. Lemonnier, G. B. Zhou, B. Prasher, M. Mukerji, Z. Chen, S. K. Brahmachari, D. Noble, C. Auffray & M. Sagner, Traditional Knowledge-based Medicine: A Review of History, Principles, and Relevance in the Present Context of P4 Systems Medicine. Prog. Prev. Med. 2017, vol. 2, e0011.

5. CrossRef

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