Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line

Author:

Bagriacik Emin Umit12,Baykaner Mustafa Kemali3,Yaman Melek12,Sivrikaya Gizem1,Durdağ Emre3,Emmez Hakan3,Fincan GökçeÖztürk4,Börcek Alp Özgün3,Seçen Ahmet Eren3,Ercan Sevim4

Affiliation:

1. Immunology Research Center, Gazi University, Ankara, Turkey

2. Immunology Department

3. Division of Pediatric Neurosurgery

4. Medical Pharmacology Department, Gazi University, Faculty of Medicine, Ankara, Turkey

Abstract

Abstract BACKGROUND Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers. OBJECTIVE To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma–bearing patients and to test their sensitivity against various anticancer chemotherapy drugs. METHODS Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with 3H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs. RESULTS We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully. CONCLUSION Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Neurology (clinical),Surgery

Reference46 articles.

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3. Astrocyte-astrocytoma cell line interactions in culture;Lal;J Neurosci Res,1996

4. Transfection of human astrocytoma cells with glial fibrillary acidic protein complementary DNA: analysis of expression, proliferation, and tumorigenicity;Rutka;Cancer Res,1993

5. Delayed occurrence of cerebellar pleomorphic xanthoastrocytoma after supratentorial pleomorphic xanthoastrocytoma removal;Glasser;Case report. J Neurosurg,1995

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