A Versatile Sub-Nanomolar Fluorescent Ligand Enables NanoBRET Binding Studies and Single-Molecule Microscopy at the Histamine H3 Receptor

Author:

Rosier Niklas1,Grätz Lukas1,Schihada Hannes2ORCID,Möller Jan34,Işbilir Ali34,Humphrys Laura J.1,Nagl Martin1,Seibel Ulla1,Lohse Martin J.345,Pockes Steffen167ORCID

Affiliation:

1. Institute of Pharmacy, University of Regensburg, Universitätsstraße 31, Regensburg 93053, Germany

2. Section of Receptor Biology & Signaling, Dept. of Physiology & Pharmacology, Karolinska Institutet, Solnavägen 1, Stockholm 171 77, Sweden

3. Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, Berlin 13125, Germany

4. Institute of Pharmacology and Toxicology and Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Straße 2, Würzburg 97070, Germany

5. ISAR Bioscience Institute, Semmelweisstraße 5, Planegg 82152, Germany

6. Department of Neurology, University of Minnesota, 2101 6th Street SE, Minneapolis, Minnesota 55455, United States

7. Department of Medicinal Chemistry, Institute for Therapeutics Discovery and Development, University of Minnesota, Minneapolis, Minnesota 55414, United States

Funder

Elitenetzwerk Bayern

Deutsche Forschungsgemeinschaft

Publisher

American Chemical Society (ACS)

Subject

Drug Discovery,Molecular Medicine

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