Simultaneous Reduction and Alkylation of Protein Disulfides in a Centrifugal Ultrafiltration Device Prior to Two-Dimensional Gel Electrophoresis
Author:
Affiliation:
1. Pressure BioSciences Incorporated, West Bridgewater, Massachusetts, Proteome Systems Incorporated, Woburn, Massachusetts, and Millipore Corporation, Billerica, Massachusetts
Publisher
American Chemical Society (ACS)
Subject
General Chemistry,Biochemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/pr050439w
Reference28 articles.
1. Ultrafiltration for proteomic sample preparation
2. Reduction and alkylation of proteins in preparation of two-dimensional map analysis: Why, when, and how?
3. β-elimination: An unexpected artefact in proteome analysis
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