De Novo Glycan Display on Cell Surfaces Using HaloTag: Visualizing the Effect of the Galectin Lattice on the Lateral Diffusion and Extracellular Vesicle Loading of Glycosylated Membrane Proteins

Author:

Miura Ayane1,Manabe Yoshiyuki12ORCID,Suzuki Kenichi G. N.34,Shomura Hiroki1,Okamura Soichiro1,Shirakawa Asuka1,Yano Kumpei1,Miyake Shuto1,Mayusumi Koki1,Lin Chun-Cheng56ORCID,Morimoto Kenta7,Ishitobi Jojiro7,Nakase Ikuhiko78,Arai Kenta9,Kobayashi Shouhei9,Ishikawa Ushio10,Kanoh Hirotaka10,Miyoshi Eiji11,Yamaji Toshiyuki12,Kabayama Kazuya1213ORCID,Fukase Koichi1214ORCID

Affiliation:

1. Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

2. Forefront Research Center, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

3. Institute for Glyco-core Research (iGCORE), Gifu University, Gifu 501-1193, Japan

4. National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan

5. Department of Chemistry, National Tsing-Hua University, Hsinchu 30013, Taiwan

6. Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

7. Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, 1-1, Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan

8. Department of Biological Chemistry, School of Science, Osaka Metropolitan University, 1-1, Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan

9. Advanced ICT Research Institute, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe, Hyogo 651-2492, Japan

10. Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, 4-4-1, Komatsushima, Aoba Ward, Sendai, Miyagi 981-8558, Japan

11. Department of Molecular Biochemistry and Clinical Investigation, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan

12. Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan

13. Interdisciplinary Research Center for Radiation Sciences, Institute for Radiation Sciences, Osaka University, 2-4 Yamadaoka, Suita, Osaka 565-0871, Japan

14. Center for Advanced Modalities and DDS, Osaka University, 1-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Funder

Core Research for Evolutional Science and Technology

Japan Agency for Medical Research and Development

Japan Society for the Promotion of Science

Fusion Oriented REsearch for disruptive Science and Technology

Publisher

American Chemical Society (ACS)

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