Multiparametric Single-Vesicle Flow Cytometry Resolves Extracellular Vesicle Heterogeneity and Reveals Selective Regulation of Biogenesis and Cargo Distribution

Author:

von Lersner Ariana K.1ORCID,Fernandes Fabiane23,Ozawa Patricia Midori Murobushi45ORCID,Jackson Marques6,Masureel Matthieu7,Ho Hoangdung7,Lima Sierra M.5,Vagner Tatyana8ORCID,Sung Bong Hwan45,Wehbe Mohamed9,Franze Kai610,Pua Heather24ORCID,Wilson John T.1249ORCID,Irish Jonathan M.125,Weaver Alissa M.1245ORCID,Di Vizio Dolores8ORCID,Zijlstra Andries1246

Affiliation:

1. Program in Cancer Biology, Vanderbilt University, Nashville, Tennessee 37232, United States

2. Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, United States

3. Institute of Applied Biosciences and Chemistry, Hogeschool Arnhem en Nijmegen University of Applied Sciences, Nijmegen 6525 EM, Gelderland, Netherlands

4. The Center for EV Research, Vanderbilt University, Nashville, Tennessee 37232, United States

5. Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, United States

6. Department of Research Pathology, Genentech, San Francisco, California 94080, United States

7. Department of Structural Biology, Genentech, San Francisco, California 94080, United States

8. Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, California 90048, United States

9. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37232, United States

10. KNIME GmbH, Konstanz 78467, Germany

Funder

Division of Cancer Prevention, National Cancer Institute

Division of Civil, Mechanical and Manufacturing Innovation

Division of Chemical, Bioengineering, Environmental, and Transport Systems

Publisher

American Chemical Society (ACS)

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