A New General Model for Predicting Melting Thermodynamics of Complementary and Mismatched B-Form Duplexes Containing Locked Nucleic Acids: Application to Probe Design for Digital PCR Detection of Somatic Mutations-Reference-Cited by-同舟云学术

A New General Model for Predicting Melting Thermodynamics of Complementary and Mismatched B-Form Duplexes Containing Locked Nucleic Acids: Application to Probe Design for Digital PCR Detection of Somatic Mutations

Published:2015-02-05 Issue:6 Volume:54 Page:1338-1352
ISSN:0006-2960
Container-title:Biochemistry
language:en
Short-container-title:Biochemistry

Author:

Hughesman Curtis¹,Fakhfakh Kareem¹²³,Bidshahri Roza¹,Lund H. Louise¹⁴,Haynes Charles¹²³⁴

Affiliation:

1. Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada V6T 1Z4

2. Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC, Canada V6T 1Z3

3. RES’EAU Water Research Network, Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC, Canada V6T 1Z3

4. Genome Science and Technology (GSAT) program, University of British Columbia, Vancouver, BC, Canada V5Z 4S6

Funder

Canadian Institutes of Health Research

Canada Research Chairs

Michael Smith Development Fund

Natural Sciences and Engineering Research Council of Canada

Publisher

American Chemical Society (ACS)

Subject

Biochemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/bi500905b

Reference59 articles.

1. Clinical genotyping: The need for interrogation of single nucleotide polymorphisms and mutations in the clinical laboratory

2. 3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

3. Evaluation of the performance of LNA and MGB probes in 5′-nuclease PCR assays

4. Real-time genotyping with oligonucleotide probes containing locked nucleic acids

5. Single nucleotide polymorphism genotyping using locked nucleic acid (LNA™)

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