Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin

Author:

Shen Yi1ORCID,Wen Yurong23,Sposini Silvia45,Vishwanath Anjali Amrapali6,Abdelfattah Ahmed S.78ORCID,Schreiter Eric R.7,Lemieux M. Joanne2ORCID,de Juan-Sanz Jaime6,Perrais David4,Campbell Robert E.19ORCID

Affiliation:

1. Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada

2. Department of Biochemistry, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

3. Center for Microbiome Research of Med-X Institute, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China

4. CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, University of Bordeaux, Bordeaux 33076, France

5. Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Imperial College London, London SW7 2BX, United Kingdom

6. Institut du Cerveau-Paris Brain Institute-ICM, Inserm, CNRS, APHP, Häpital de la Pitié Salpêtrière, Sorbonne Université, 75013 Paris, France

7. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virgina 20147, United States

8. Department of Neuroscience, Brown University, Providence, Rhode Island 02906, United States

9. Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

Funder

Howard Hughes Medical Institute

Canadian Institutes of Health Research

Agence Nationale de la Recherche

Parkinson Association of Alberta

Wellcome Trust

Natural Sciences and Engineering Research Council of Canada

National Natural Science Foundation of China

Publisher

American Chemical Society (ACS)

Subject

Fluid Flow and Transfer Processes,Process Chemistry and Technology,Instrumentation,Bioengineering

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