Development of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons

Author:

Chai Fu1,Fujii Hajime2,Le Giang N. T.3,Lin Chang4ORCID,Ota Keisuke2,Lin Karl Matthew1,Pham Lam M. T.3,Zou Peng4ORCID,Drobizhev Mikhail5,Nasu Yusuke16ORCID,Terai Takuya1ORCID,Bito Haruhiko2,Campbell Robert E.17ORCID

Affiliation:

1. Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan

2. Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan

3. Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada

4. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

5. Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, United States

6. PRESTO, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0075, Japan

7. CERVO Brain Research Center and Department of Biochemistry, Microbiology, and Bioinformatics, Université Laval, Québec, Québec G1 V 0A6, Canada

Funder

Ministry of Science and Technology of the People's Republic of China

BRAIN Initiative

Japan Agency for Medical Research and Development

Fulbright U.S. Student Program

Japan Society for the Promotion of Science

Japan Science and Technology Agency

Natural Sciences and Engineering Research Council of Canada

National Natural Science Foundation of China

Japan-U.S. Education Commission

Publisher

American Chemical Society (ACS)

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