Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis-Reference-Cited by-同舟云学术

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Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis

Published:2012-06-22 Issue:2 Volume:2 Page:83-92
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Kille Sabrina¹²,Acevedo-Rocha Carlos G.¹²,Parra Loreto P.¹²,Zhang Zhi-Gang¹²,Opperman Diederik J.¹,Reetz Manfred T.¹²,Acevedo Juan Pablo³

Affiliation:

1. Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany

2. Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße, 35043 Marburg, Germany

3. Facultad de Medicina y Facultad de Ingeniería de la Universidad de los Andes, Santiago, Chile

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/sb300037w

Reference65 articles.

1. Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

2. A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides

3. Cloning of random-sequence oligodeoxynucleotides

4. Exploring and designing protein function with restricted diversity

5. Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

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