Label-Free Fluorescence Quantification of Hydrolytic Enzyme Activity on Native Substrates Reveals How Lipase Function Depends on Membrane Curvature

Author:

Bohr Søren S.-R.12ORCID,Thorlaksen Camilla1234,Kühnel Ronja Marie5,Günther-Pomorski Thomas56,Hatzakis Nikos S.12ORCID

Affiliation:

1. Department of Chemistry & Nanoscience Center, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C 1871, Denmark

2. Novo Nordisk Center for Protein Research (CPR), University of Copenhagen, Blegdamsvej 3B, Copenhagen 2200, Denmark

3. Biophysics, Novo Nordisk A/S, Novo Nordisk Park 1, Maaloev 2760, Denmark

4. Drug Delivery and Biophysics of Biopharmaceuticals, Department of Pharmacy, University of Copenhagen, Universitetsparken 2, Copenhagen 2100, Denmark

5. Faculty of Chemistry and Biochemistry, Department of Molecular Biochemistry, Ruhr University Bochum, Universitätstrasse 150, D-44780 Bochum, Germany

6. Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C 1871, Denmark

Funder

Novo Nordisk Fonden

Villum Fonden

Carlsbergfondet

Publisher

American Chemical Society (ACS)

Subject

Electrochemistry,Spectroscopy,Surfaces and Interfaces,Condensed Matter Physics,General Materials Science

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