An Approach to the Precise Chemoenzymatic Synthesis of 13C-Labeled Sialyloligosaccharide on an Intact Glycoprotein:  A Novel One-Pot [3-13C]-Labeling Method for Sialic Acid Analogues by Control of the Reversible Aldolase Reaction, Enzymatic Synthesis of [3-13C]-NeuAc-α-(2→3)-[U-13C]-Gal-β-(1→4)-GlcNAc-β- Sequence onto Glycoprotein, and Its Conformational Analysis by Developed NMR Techniques

Author:

Miyazaki Tatsuo1,Sato Hajime1,Sakakibara Tohru1,Kajihara Yasuhiro1

Affiliation:

1. Contribution from the Graduate School of Integrated Science, and Faculty of Science, Yokohama City University, 22-2, Seto, Kanazawa-ku, Yokohama 236-0027 Japan, and Bruker Japan Co., Ltd. 21-5, Ninomiya, 3-chome, Tsukuba, Ibaraki 305-0051 Japan

Publisher

American Chemical Society (ACS)

Subject

Colloid and Surface Chemistry,Biochemistry,General Chemistry,Catalysis

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