An Approach to the Precise Chemoenzymatic Synthesis of 13C-Labeled Sialyloligosaccharide on an Intact Glycoprotein: A Novel One-Pot [3-13C]-Labeling Method for Sialic Acid Analogues by Control of the Reversible Aldolase Reaction, Enzymatic Synthesis of [3-13C]-NeuAc-α-(2→3)-[U-13C]-Gal-β-(1→4)-GlcNAc-β- Sequence onto Glycoprotein, and Its Conformational Analysis by Developed NMR Techniques
Author:
Affiliation:
1. Contribution from the Graduate School of Integrated Science, and Faculty of Science, Yokohama City University, 22-2, Seto, Kanazawa-ku, Yokohama 236-0027 Japan, and Bruker Japan Co., Ltd. 21-5, Ninomiya, 3-chome, Tsukuba, Ibaraki 305-0051 Japan
Publisher
American Chemical Society (ACS)
Subject
Colloid and Surface Chemistry,Biochemistry,General Chemistry,Catalysis
Link
https://pubs.acs.org/doi/pdf/10.1021/ja994211j
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