Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification–Mass Spectrometry-Reference-Cited by-同舟云学术

Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification–Mass Spectrometry

Published:2023-09-15 Issue:10 Volume:22 Page:3383-3391
ISSN:1535-3893
Container-title:Journal of Proteome Research
language:en
Short-container-title:J. Proteome Res.

Author:

Hollenstein David M.¹²^ORCID,Maurer-Granofszky Margarita³,Reiter Wolfgang²^ORCID,Anrather Dorothea²,Gossenreiter Thomas²,Babic Riccardo⁴⁵⁶^ORCID,Hartl Natascha¹²,Kraft Claudine⁴⁷,Hartl Markus¹²^ORCID

Affiliation:

1. Department for Biochemistry and Cell Biology, University of Vienna, Center for Molecular Biology, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna 1030, Austria

2. Mass Spectrometry Facility, Max Perutz Laboratories, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 7, Vienna 1030, Austria

3. St. Anna Children’s Cancer Research Institute (CCRI), Zimmermannplatz 10, Vienna 1090, Austria

4. Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Freiburg 79104, Germany

5. Faculty of Biology, University of Freiburg, Freiburg 79104, Germany

6. Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg 79104, Germany

7. CIBSS - Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg 79104, Germany

Funder

Austrian Science Fund

Deutsche Forschungsgemeinschaft

H2020 European Research Council

Publisher

American Chemical Society (ACS)

Subject

General Chemistry,Biochemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.3c00424

Reference24 articles.

1. A comprehensive comparison between camelid nanobodies and single chain variable fragments

2. OpenCell: Endogenous tagging for the cartography of human cellular organization

3. Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins

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