Toward Zero Variance in Proteomics Sample Preparation: Positive-Pressure FASP in 96-Well Format (PF96) Enables Highly Reproducible, Time- and Cost-Efficient Analysis of Sample Cohorts-Reference-Cited by-同舟云学术

Toward Zero Variance in Proteomics Sample Preparation: Positive-Pressure FASP in 96-Well Format (PF96) Enables Highly Reproducible, Time- and Cost-Efficient Analysis of Sample Cohorts

Published:2022-03-22 Issue:4 Volume:21 Page:1181-1188
ISSN:1535-3893
Container-title:Journal of Proteome Research
language:en
Short-container-title:J. Proteome Res.

Author:

Loroch Stefan¹^ORCID,Kopczynski Dominik¹,Schneider Adriana C.¹²,Schumbrutzki Cornelia¹,Feldmann Ingo¹,Panagiotidis Eleftherios¹,Reinders Yvonne¹,Sakson Roman¹^ORCID,Solari Fiorella A.¹,Vening Alicia³,Swieringa Frauke³,Heemskerk Johan W. M.³,Grandoch Maria⁴,Dandekar Thomas⁵^ORCID,Sickmann Albert¹⁶⁷^ORCID

Affiliation:

1. Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., 44139 Dortmund, Germany

2. Faculty of Biochemical and Chemical Engineering, Technical University of Dortmund, 44227 Dortmund, Germany

3. Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 6200 MD Maastricht, The Netherlands

4. Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum der Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany

5. Department of Bioinformatics, Biocenter, University of Würzburg, 97074 Würzburg, Germany

6. Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany

7. Department of Chemistry, College of Physical Sciences, University of Aberdeen, AB24 3FX Aberdeen, United Kingdom

Funder

Bundesministerium f?r Bildung und Forschung

Deutsche Forschungsgemeinschaft

Ministerium f?r Kultur und Wissenschaft des Landes Nordrhein-Westfalen

Regierender B?rgermeister von Berlin

Publisher

American Chemical Society (ACS)

Subject

General Chemistry,Biochemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.1c00706

Reference31 articles.

1. Parallel Accumulation–Serial Fragmentation (PASEF): Multiplying Sequencing Speed and Sensitivity by Synchronized Scans in a Trapped Ion Mobility Device

2. diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition

3. BoxCar acquisition method enables single-shot proteomics at a depth of 10,000 proteins in 100 minutes

4. A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients

5. A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics

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