Comprehensive N- and O-Glycoproteomic Analysis of Multiple Chinese Hamster Ovary Host Cell Lines

Author:

Wang Qiong12ORCID,Wang Tiexin2,Wu Wells W.3,Lin Chang-Yi3,Yang Shuang14ORCID,Yang Ganglong56ORCID,Jankowska Ewa1,Hu Yifeng2,Shen Rong-Fong3,Betenbaugh Michael J.2ORCID,Cipollo John F.1ORCID

Affiliation:

1. Laboratory of Bacterial Polysaccharides, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993, United States

2. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21210, United States

3. Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993, United States

4. Center for Clinical Mass Spectrometry, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China

5. Department of Pathology, Johns Hopkins University, Baltimore, Maryland 21287, United States

6. Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China

Funder

Food and Drug Administration

National Science Foundation

Publisher

American Chemical Society (ACS)

Subject

General Chemistry,Biochemistry

Reference67 articles.

1. Kattla, J. Biopharmaceuticals─Current FDA & EMAs Regulations on Glycan Analysis, 2015. https://www.waters.com/webassets/cms/library/docs/local_seminar_presentations/SW%20FDAs%20regulation%20on%20protein%20glycosylation%20-%20Jayesh%20Kattla.pdf. (Accessed July 3, 2020).

2. Optimisation of the Cellular Metabolism of Glycosylation for Recombinant Proteins Produced by Mammalian Cell Systems

3. The choice of mammalian cell host and possibilities for glycosylation engineering

4. Production of Recombinant Proteins: Challenges and Solutions

5. Post-translational modifications in the context of therapeutic proteins

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