Improved Process for the Preparation of Nucleosidic Phosphoramidites Using a Safer and Cheaper Activator
Author:
Affiliation:
1. Manufacturing Process Department, Isis Pharmaceuticals, Inc., 2292 Faraday Avenue, Carlsbad, California 92008, U.S.A.
Publisher
American Chemical Society (ACS)
Subject
Organic Chemistry,Physical and Theoretical Chemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/op990086k
Reference26 articles.
1. Sanghvi, Y. S.; Andrade, M.; Deshmukh, R. R.; Holmburg, L.; Scozzari, A. N.; Cole, D. L. InManual of Antisense Methodology; Hartmann, G., Endres, S., Eds. Kluwer Academic Publishers: Norwell, MA, 1999; Chapter 1, p 3.
2. Polymer support oligonucleotide synthesis XVIII1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product
3. In situactivation of bis-dialkylaminophosphines—a new method for synthesizing deoxyoligonucleotides on polymer supports
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