Specific Photoaffinity-Labeling of Tyr-50 on the Heavy Chain and of Tyr-32 on the Light Chain in the Steroid Combining Site of a Mouse Monoclonal Anti-Estradiol Antibody Using C3-, C6-, and C7-Linked 5-Azido-2-nitrobenzoylamidoestradiol Photoreagents-Reference-Cited by-同舟云学术

Specific Photoaffinity-Labeling of Tyr-50 on the Heavy Chain and of Tyr-32 on the Light Chain in the Steroid Combining Site of a Mouse Monoclonal Anti-Estradiol Antibody Using C3-, C6-, and C7-Linked 5-Azido-2-nitrobenzoylamidoestradiol Photoreagents

Published:2001-11-15 Issue:49 Volume:40 Page:14907-14920
ISSN:0006-2960
Container-title:Biochemistry
language:en
Short-container-title:Biochemistry

Author:

Rolland de Ravel Marc¹,Blachère Thierry¹,Delolme Frédéric¹,Dessalces Guy¹,Coulon Stéphane¹,Baty Daniel¹,Grenot Catherine¹,Mappus Elisabeth¹,Cuilleron Claude Y.¹

Affiliation:

1. Institut National de la Santé et de la Recherche Médicale, Unité INSERM U 329, Pathologie Hormonale Moléculaire, Hôpital Debrousse, 69322 Lyon, France, Service Central d'Analyse, SCA-CNRS-USR59, 69390 Vernaison, France, and Institut de Biologie Structurale et de Microbiologie, Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UPR 9027, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille, France

Publisher

American Chemical Society (ACS)

Subject

Biochemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/bi011174b

Reference44 articles.

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1. Two-Step in Vitro Antibody Affinity Maturation Enables Estradiol-17β Assays with More than 10-Fold Higher Sensitivity;Analytical Chemistry;2010-01-04

2. Functional characterization of an anti-estradiol antibody by site-directed mutagenesis and molecular modelling: modulation of binding properties and prominent role of the VLdomain in estradiol recognition;Journal of Molecular Recognition;2002-01