Continuous Online Titer Monitoring in CHO Cell Culture Supernatant Using a Herringbone Nanofluidic Filter Array

Author:

Rohskopf Zhumei12ORCID,Kwon Taehong23ORCID,Ko Sung Hee23,Bozinovski Dragana4,Jeon Hyungkook2ORCID,Mohan Naresh4,Springs Stacy L.45ORCID,Han Jongyoon2365

Affiliation:

1. Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

2. Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

3. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge,Massachusetts 02139, United States

4. Center for Biomedical Innovation, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

5. Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, Singapore-MIT Alliance for Research and Technology (SMART) Centre, Singapore117583,Singapore

6. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States

Funder

U.S. Food and Drug Administration

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Reference46 articles.

1. Production of recombinant protein therapeutics in cultivated mammalian cells

2. FDA. Guidance for Industry PAT - A Framework for Innovative Pharmaceutical Development, Manufacturing, and Quality Assurance; 2004. https://www.fda.gov/media/71012/download (accessed 2022 – 09 – 14).

3. The Current Scientific and Regulatory Landscape in Advancing Integrated Continuous Biopharmaceutical Manufacturing

4. In-line and real-time prediction of recombinant antibody titer by in situ Raman spectroscopy

5. Czeterko, M.; Debaise, A.; Pierce, W.; Conway, M. In Situ Raman Spectroscopy Systems and Methods for Controlling Process Variables in Cell Cultures. US20190112569A1, October 15, 2019. https://patents.google.com/patent/US20190112569A1/en (accessed 2022 – 12 – 14).

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